Oligos in Action
2024
INT-1B3, an LNP formulated miR-193a-3p mimic, promotes anti-tumor immunity by enhancing T cell mediated immune responses via modulation of the tumor microenvironment and induction of immunogenic cell death
Duurland, C. L., de Gunst, T., den Boer, H. C., van den Bosch, M. T. J., Telford, B. J., Vos, R. M., Xie, X., Zang, M., Wang, F., Shao, Y., An, X., Wang, J., Cai, J., Bourré, L., van Pinxteren, L. A. H., Schaapveld, R. Q. J., Janicot, M., Yahyanejad, S.
Role of the soluble epoxide hydrolase in keratinocyte proliferation and sensitivity of skin to inflammatory stimuli
Naeem, Z., Zukunft, S., Huard, A., Hu, J., Hammock, B. D., Weigert, A., Frömel, T., Fleming, I.
Biomedicine & Pharmacotherapy Vol. 171 (2024)
Profiling the interactome of oligonucleotide drugs by proximity biotinylation
Hanswillemenke, A., Hofacker, D. T., Sorgenfrei, M., Fruhner, C., Franz-Wachtel, M., Schwarzer, D., Maček, B., Stafforst, T.
A pipeline for identifying guide RNA sequences that promote RNA editing of nonsense mutations that cause inherited retinal diseases
Schneider, N., Steinberg, R., Ben-David, A., Valensi, J., David-Kadoch, G., Rosenwasser, Z., Banin, E., Levanon, E. Y., Sharon, D., Ben-Aroya, S.
Molecular Therapy - Nucleic Acids (2024)
GNTI-122: an autologous antigen-specific engineered Treg cell therapy for type 1 diabetes
Uenishi, G. I., Repic, M., Yam, J. Y., Landuyt, A., Saikumar-Lakshmi, P., Guo, T., Zarin, P., Sassone-Corsi, M., Chicoine, A., Kellogg, H., Hunt, M., Drow, T., Tewari, R., Cook, P. J., Yang, S. J., Cerosaletti, K., Schweinoch, D., Guiastrennec, B., James, E., Patel, C., Chen, T. F., Buckner, J. H., Rawlings, D. J., Wickham, T. J., Mueller, K. T.
Add-on multiple submucosal injections of the RNA oligonucleotide GUT-1 to anti-TNF antibody treatment in patients with moderate-to-severe ulcerative colitis: an open-label, proof-of concept study
Suzuki, K., Sameshima, Y., Yokoyama, J., Terai, S., Yoneyama, H., Atreya, R., Neurath, M. F., Hibi, T., Asakura, H.
The tumor suppressor 5A2, a synthetic miR-7-5p mimic, targets oncogenic and metabolic pathways, as revealed by transcriptome-wide analysis
van den Bosch, M. T. J., Telford, B. J., Yahyanejad, S., de Gunst, T., den Boer, H. C., Vos, R. M., Duurland, C. L., Biemans, R., Dubois, L. J., van Pinxteren, L. A. H., Schaapveld, R. Q. J., Janicot, M.
Frontiers in Drug Discovery Vol. 3 (2023)
Cationic LNP-formulated mRNA expressing Tie2-agonist in the lung endothelium prevents pulmonary vascular leakage
Radloff, K., Gutbier, B., Dunne, C. M., Moradian, H., Schwestka, M., Gossen, M., Ahrens, K., Kneller, L., Wang, Y., Moga, A., Gkionis, L., Keil, O., Fehring, V., Tondera, D., Giese, K., Santel, A., Kaufmann, J., Witzenrath, M.
Molecular Therapy: Nucleic Acids Vol. 34 (2023)
Targeted siRNA lipid nanoparticles for the treatment of KRAS-mutant tumors
Anthiya, S., Öztürk, S.C., Yanik, H., Tavukcuoglu, E., Şahin, A., Datta, D., Charisse, K., Álvarez, D.M., Loza, M.I., Calvo, A., & Sulheim, E.
Journal of Controlled Release (2023)
ADAR1-mediated RNA editing promotes B cell lymphomagenesis
Pecori, R., Ren, W., Pirmoradian, M., Wang, X., Liu, D., Berglund, M., Li, W., Tasakis, R. N., Di Giorgio, S., Ye, X., Li, X., Arnold, A., Wüst, S., Schneider, M., Selvasaravanan, K.-D., Fuell, Y., Stafforst, T., Amini, R.-M., Sonnevi, K., Enblad, G., Sander, B., Wahlin, B. E., Wu, K., Zhang, H., Helm, D., Binder, M., Papavasiliou, F. N., Pan-Hammarström, Q.
Combination of protein and cell internalization SELEX identifies a potential RNA therapeutic and delivery platform to treat EphA2-expressing tumors
Santana-Viera, L., Dassie, J. P., Rosàs-Lapeña, M., Garcia-Monclús, S., Chicón-Bosch, M., Pérez-Capó, M., del Pozo, L., Sanchez-Serra, S., Almacellas-Rabaiget, O., Maqueda-Marcos, S., López-Alemany, R., Thiel, W. H., Giangrande, P. H., Tirado, O. M.
Positron Emission Tomography Studies of the Biodistribution, Translocation, and Fate of Poly Allyl Amine-Based Carriers for siRNA Delivery by Systemic and Intratumoral Administration
Simo, C., Salvador, C., Andreozzi, P., Gomez-Vallejo, V., Romero, G., Dupin, D., Llop, J., Moya, S. E.
Spacer Fidelity Assessments of Guide RNA by Top-Down Mass Spectrometry
Macias, L. A., Garcia, S. P., Back, K. M., Wu, Y., Johnson, G. H., Kathiresan, S., Bellinger, A. M., Rohde, E., Freitas, M. A., Madsen, J. A.
An Aptamer against MNK1 for Non-Small Cell Lung Cancer Treatment
Carrión-Marchante, R., Pinto-Díez, C., Klett-Mingo, J. I., Palacios, E., Barragán-Usero, M., Pérez-Morgado, M. I., Pascual-Mellado, M., Alcalá, S., Ruiz-Cañas, L., Sainz, B., Jr., González, V. M., Martín, M. E.
Correlating the Structure and Gene Silencing Activity of Oligonucleotide-Loaded Lipid Nanoparticles Using Small-Angle X-ray Scattering
Hammel, M., Fan, Y., Sarode, A., Byrnes, A. E., Zang, N., Kou, P., Nagapudi, K., Leung, D., Hoogenraad, C. C., Chen, T., Yen, C.-W., Hura, G. L.
Herpes simplex virus PCR in 2230 explanted corneal buttons
Tóth, G., Berkó-Göttel, B., Seitz, B., Langenbucher, A., Stachon, T., Pluzsik, M.T., Nagy, Z.Z., Smola, S., & Szentmáry, N.
Selective Targeting and Eradication of Various Human Non-Small Cell Lung Cancer Cell Lines Using Self-Assembled Aptamer-Decorated Nanoparticles
Barak, D., Engelberg, S., Assaraf, Y. G., Livney, Y. D.
Targeting TGF-ß in the Central Nervous System: Assessment of Cynomolgus Monkey—Toxicity and Pharmacokinetics for an LNA-Antisense Oligonucleotide
Peters, S., Wirkert, E., Kuespert, S., Heydn, R., Korte, S., Mecklenburg, L., Aigner, L., Johannesen, S., Bruun, T.-H., Bogdahn, U.
Predictive high-throughput screening of PEGylated lipids in oligonucleotide-loaded lipid nanoparticles for neuronal gene silencing
Sarode, A., Fan, Y., Byrnes, A. E., Hammel, M., Hura, G. L., Fu, Y., Kou, P., Hu, C., Hinz, F. I., Roberts, J., Koenig, S. G., Nagapudi, K., Hoogenraad, C. C., Chen, T., Leung, D., Yen, C.-W.
Enhancement of protective efficacy of innate immunostimulant based formulations against yolk sac infection in young chicks
Nguyen, T. T. T., Shahin, K., Allan, B., Sarfraz, M., Wheler, C., Gerdts, V., Köster, W., Dar, A.
Functional Downregulation of PD-L1 and PD-L2 by CpG and non-CpG Oligonucleotides in Melanoma Cells
Kleemann, J., Steinhorst, K., König, V., Zöller, N., Cinatl, J., Jr., Özistanbullu, D., Kaufmann, R., Meissner, M., Kippenberger, S.
SAMHD1 controls innate immunity by regulating condensation of immunogenic self RNA
Maharana, S., Kretschmer, S., Hunger, S., Yan, X., Kuster, D., Traikov, S., Zillinger, T., Gentzel, M., Elangovan, S., Dasgupta, P., Chappidi, N., Lucas, N., Maser, K. I., Maatz, H., Rapp, A., Marchand, V., Chang, Y.-T., Motorin, Y., Hubner, N., Hartmann, G., Hyman, A. A., Alberti, S., Lee-Kirsch, M. A.
Optimized Hemolysin Type 1 Secretion System in Escherichia coli by Directed Evolution of the Hly Enhancer Fragment and Including a Terminator Region
Pourhassan N., Z., Cui, H., Khosa, S., Davari, M. D., Jaeger, K.-E., Smits, S. H. J., Schwaneberg, U., Schmitt, L.
In vivo base editing rescues cone photoreceptors in a mouse model of early-onset inherited retinal degeneration
Choi, E. H., Suh, S., Foik, A. T., Leinonen, H., Newby, G. A., Gao, X. D., Banskota, S., Hoang, T., Du, S. W., Dong, Z., Raguram, A., Kohli, S., Blackshaw, S., Lyon, D. C., Liu, D. R., Palczewski, K.
RNA-Cholesterol Nanoparticles Function as Potent Immune Activators via TLR7 and TLR8
Obermann, H.-L., Lederbogen, I. I., Steele, J., Dorna, J., Sander, L. E., Engelhardt, K., Bakowsky, U., Kaufmann, A., Bauer, S.
Base editing of hematopoietic stem cells rescues sickle cell disease in mice
Newby, G. A., Yen, J. S., Woodard, K. J., Mayuranathan, T., Lazzarotto, C. R., Li, Y., Sheppard-Tillman, H., Porter, S. N., Yao, Y., Mayberry, K., Everette, K. A., Jang, Y., Podracky, C. J., Thaman, E., Lechauve, C., Sharma, A., Henderson, J. M., Richter, M. F., Zhao, K. T., Miller, S. M., Wang, T., Koblan, L. W., McCaffrey, A. P., Tisdale, J. F., Kalfa, T. A., Pruett-Miller, S. M., Tsai, S. Q., Weiss, M. J., Liu, D. R.
Aptamer BC 007’s affinity to specific and less-specific anti-Sars-CoV-2 neutralizing antibodies
Haberland, A., Krylova, O., Nikolenko, H., Göttel, P., Dallmann, A., Müller, J., & Weisshoff, H.
The consequences of soluble epoxide hydrolase deletion on tumorigenesis and metastasis in a mouse model of breast cancer
Kesavan, R., Frömel, T., Zukunft, S., Brüne, B., Weigert, A., Wittig, I., Popp, R., & Fleming, I.
International Journal of Molecular Sciences (2021)
Regulation of GC box activity by 8-oxoguanine.
Müller, N. & Khobta, A.
Targeted Nanoparticles Harboring Jasmine-Oil-Entrapped Paclitaxel for Elimination of Lung Cancer Cells
Engelberg, S., Lin, Y., Assaraf, Y. G., Livney, Y. D.
Overcoming PD-1 Blockade Resistance with CpG-A Toll-Like Receptor 9 Agonist Vidutolimod in Patients with Metastatic Melanoma
Ribas, A., Medina, T., Kirkwood, J. M., Zakharia, Y., Gonzalez, R., Davar, D., Chmielowski, B., Campbell, K. M., Bao, R., Kelley, H., Morris, A., Mauro, D., Wooldridge, J. E., Luke, J. J., Weiner, G. J., Krieg, A. M., Milhem, M. M.
Multi-modal effects of 1B3, a novel synthetic miR-193a-3p mimic, support strong potential for therapeutic intervention in oncology
Telford, B. J., Yahyanejad, S., de Gunst, T., den Boer, H. C., Vos, R. M., Stegink, M., van den Bosch, M. T. J., Alemdehy, M. F., van Pinxteren, L. A. H., Schaapveld, R. Q. J., Janicot, M.
Transcriptome-wide analysis reveals insight into tumor suppressor functions of 1B3, a novel synthetic miR-193a-3p mimic
van den Bosch, M. T. J., Yahyanejad, S., Alemdehy, M. F., Telford, B. J., de Gunst, T., den Boer, H. C., Vos, R. M., Stegink, M., van Pinxteren, L. A. H., Schaapveld, R. Q. J., Janicot, M.
Molecular Therapy: Nucleic Acids (2021)
CroSR391, an ortholog of the λ Cro repressor, plays a major role in suppressing polVR391-dependent mutagenesis
McDonald, J. P., Quiros, D. R., Vaisman, A., Mendez, A. R., Reyelt, J., Schmidt, M., Gonzalez, M., Woodgate, R.
Direct and Base Excision Repair-Mediated Regulation of a GC-Rich cis-Element in Response to 5-Formylcytosine and 5-Carboxycytosine
Müller, N., Ponkkonen, E., Carell, T., Khobta, A.
Induced dendritic cells co-expressing GM-CSF/IFN-α/tWT1 priming T and B cells and automated manufacturing to boost GvL
Bialek-Waldmann, J. K., Domning, S., Esser, R., Glienke, W., Mertens, M., Aleksandrova, K., Arseniev, L., Kumar, S., Schneider, A., Koenig, J., Theobald, S. J., Tsay, H.-C., Cornelius, A. D. A., Bonifacius, A., Eiz-Vesper, B., Figueiredo, C., Schaudien, D., Talbot, S. R., Bleich, A., Spineli, L. M., von Kaisenberg, C., Clark, C., Blasczyk, R., Heuser, M., Ganser, A., Köhl, U., Farzaneh, F., Stripecke, R.
Molecular Therapy: Methods & Clinical Development (2021)
Sulfonated Amphiphilic Poly(α)glutamate Amine—A Potential siRNA Nanocarrier for the Treatment of Both Chemo-Sensitive and Chemo-Resistant Glioblastoma Tumors
Krivitsky, A., Pozzi, S., Yeini, E., Israeli Dangoor, S., Zur, T., Golan, S., Krivitsky, V., Albeck, N., Pisarevsky, E., Ofek, P., Madi, A., Satchi-Fainaro, R.
Embryonic stem cells are devoid of macropinocytosis, a trafficking pathway for activin A in differentiated cells
Kostopoulou, N., Bellou, S., Bagli, E., Markou, M., Kostaras, E., Hyvönen, M., Kalaidzidis, Y., Papadopoulos, A., Chalmantzi, V., Kyrkou, A., Panopoulou, E., Fotsis, T., Murphy, C.
Endothelial Tpl2 regulates vascular barrier function via JNK-mediated degradation of claudin-5 promoting neuroinflammation or tumor metastasis
Nanou, A., Bourbouli, M., Vetrano, S., Schaeper, U., Ley, S., Kollias, G.
A Bovine Enteric Mycobacterium Infection Model to Analyze Parenteral Vaccine-Induced Mucosal Immunity and Accelerate Vaccine Discovery
Facciuolo, A., Lee, A.H., Trimble, M.J., Rawlyk, N., Townsend, H.G., Bains, M., Arsic, N., Mutharia, L.M., Potter, A., Gerdts, V., & Napper, S.
Frontiers in Immunology (2020)
Antisense oligonucleotide in LNA-gapmer design targeting TGFBR2—a key single gene target for safe and effective inhibition of TGFβ signaling
Kuespert, S., Heydn, R., Peters, S., Wirkert, E., Meyer, A.L., Siebörger, M., Johannesen, S., Aigner, L., Bogdahn, U. and Bruun, T.H.
International Journal of Molecular Sciences (2020)
A three-part, randomised study to investigate the safety, tolerability, pharmacokinetics and mode of action of BC 007, neutraliser of pathogenic autoantibodies against G-protein coupled receptors in healthy, young and elderly subjects.
Becker, N.P., Haberland, A., Wenzel, K., Göttel, P., Wallukat, G., Davideit, H., Schulze-Rothe, S., Hönicke, A.S., Schimke, I., Bartel, S. & Grossmann, M.
Clinical Drug Investigation (2020)
EGFP reporters for direct and sensitive detection of mutagenic bypass of DNA lesions
Rodriguez-Alvarez, M., Kim, D., & Khobta, A.
Macrophages produce and functionally respond to interleukin-34 in colon cancer
Franzè, E., Laudisi, F., Di Grazia, A., Marônek, M., Bellato, V., Sica, G., Monteleone, G.
Radiolabeling and PET–MRI microdosing of the experimental cancer therapeutic, MN-anti-miR10b, demonstrates delivery to metastatic lesions in a murine model of metastatic breast cancer
Le Fur, M., Ross, A., Pantazopoulos, P., Rotile, N., Zhou, I., Caravan, P., Medarova, Z., Yoo, B.
Aptamer BC 007 - Efficient binder of spreading-crucial SARS-CoV-2 proteins
Weisshoff, H., Krylova, O., Nikolenko, H., Düngen, H.-D., Dallmann, A., Becker, S., Göttel, P., Müller, J., Haberland, A.
Dose-Finding Study and Pharmacokinetics Profile of the Novel 13-Mer Antisense miR-221 Inhibitor in Sprague-Dawley Rats
Di Martino, M. T., Arbitrio, M., Caracciolo, D., Scionti, F., Tagliaferri, P., Tassone, P.
Molecular Therapy: Nucleic Acids (2020)
MRI-based molecular imaging of epicardium-derived stromal cells (EpiSC) by peptide-mediated active targeting
Straub, T., Nave, J., Bouvain, P., Akbarzadeh, M., Dasa, S. S. K., Kistner, J., Ding, Z., Marzoq, A., Stepanow, S., Becker, K., Hesse, J., Köhrer, K., Flögel, U., Ahmadian, M. R., French, B. A., Schrader, J., Temme, S.
RNA and DNA Binding Epitopes of the Cold Shock Protein TmCsp from the Hyperthermophile Thermotoga maritima
von König, K., Kachel, N., Kalbitzer, H. R., Kremer, W.
Allometric scaling approaches for predicting human pharmacokinetic of a locked nucleic acid oligonucleotide targeting cancer-associated miR-221
Di Martino, M.T., Arbitrio, M., Fonsi, M., Erratico, C.A., Scionti, F., Caracciolo, D., Tagliaferri, P. & Tassone, P.
Effects of siRNA-mediated knockdown of GSK3β on retinal ganglion cell survival and neurite/axon growth
Ahmed, Z., Morgan-Warren, P.J., Berry, M., Scott, R.A.H., & Logan, A.
Comparison of PEGylated and non-PEGylated proticles: An in vitro and in vivo study
Fresacher, K., Helbok, A., Reiser, M., Blass, S., Rangger, C., Mair, C., von Guggenberg, E., Decristoforo, C., Andreae, F., & Zimmer, A.
European Journal of Pharmaceutical Sciences (2019)
Nucleotide excision repair of abasic DNA lesions
Kitsera, N., Rodriguez-Alvarez, M., Emmert, S., Carell, T., & Khobta, A.
Effect of an intravitreal antisense oligonucleotide on vision in Leber congenital amaurosis due to a photoreceptor cilium defect
Cideciyan, A.V., Jacobson, S.G., Drack, A.V., Ho, A.C., Charng, J., Garafalo, A.V., Roman, A.J., Sumaroka, A., Han, I.C., Hochstedler, M.D. & Pfeifer, W.L.
Selective eradication of human non-small cell lung cancer cells using aptamer-decorated nanoparticles harboring a cytotoxic drug cargo
Engelberg, S., Netzer, E., Assaraf, Y. G., Livney, Y. D.
EnanDIM - a novel family of L-nucleotide-protected TLR9 agonists for cancer immunotherapy
Kapp, K., Volz, B., Curran, M. A., Oswald, D., Wittig, B., Schmidt, M.
j. immunotherapy cancer (2019)
Macrophages Are a Potent Source of Streptococcus-Induced IFN-β
Feuerstein, R., Gres, V., Elias Perdigó, N., Baasch, S., Freudenhammer, M., Elling, R., Henneke, P.
Evaluation of eluforsen, a novel RNA oligonucleotide for restoration of CFTR function in in vitro and murine models of p.Phe508del cystic fibrosis
Beumer, W., Swildens, J., Leal, T., Noel, S., Anthonijsz, H., van der Horst, G., Kuiperij-Boersma, H., Potman, M., van Putten, C., Biasutto, P., Platenburg, G., de Jonge, H., Henig, N., Ritsema, T.
Enzyme-free ligation of dimers and trimers to RNA primers
Sosson, M., Pfeffer, D., Richert, C.
Epigenetic control of the angiotensin-converting enzyme in endothelial cells during inflammation
Mudersbach, T., Siuda, D., Kohlstedt, K., Fleming, I.
UGCG influences glutamine metabolism of breast cancer cells
Schömel, N., Hancock, S. E., Gruber, L., Olzomer, E. M., Byrne, F. L., Shah, D., Hoehn, K. L., Turner, N., Grösch, S., Geisslinger, G., Wegner, M.-S.
Site-Specific Cleavage of RNAs Derived from the PIM1 3′-UTR by a Metal-Free Artificial Ribonuclease
Zellmann, F., Thomas, L., Scheffer, U., Hartmann, R. K., Göbel, M. W.
Myeloid-Specific Deletion of the AMPKα2 Subunit Alters Monocyte Protein Expression and Atherogenesis
Fisslthaler, B., Zippel, N., Abdel Malik, R., Delgado Lagos, F., Zukunft, S., Thoele, J., Siuda, D., Soehnlein, O., Wittig, I., Heidler, J., Weigert, A., Fleming, I.
VEGF-Trap is a potent modulator of vasoregenerative responses and protects dopaminergic amacrine network integrity in degenerative ischemic neovascular retinopathy
Rojo Arias, J. E., Economopoulou, M., Juárez López, D. A., Kurzbach, A., Au Yeung, K. H., Englmaier, V., Merdausl, M., Schaarschmidt, M., Ader, M., Morawietz, H., Funk, R. H. W., Jászai, J.
Stable depletion of RUNX1-ETO in Kasumi-1 cells induces expression and enhanced proteolytic activity of Cathepsin G and Neutrophil Elastase
Schoenherr, C., Wohlan, K., Dallmann, I., Pich, A., Hegermann, J., Ganser, A., Hilfiker-Kleiner, D., Heidenreich, O., Scherr, M., Eder, M.